![]() coli strain that carries the Q5 High-Fidelity DNA Polymerase gene. All reactions were conducted using 30 cycles of amplification and visualized by microfluidic LabChip ® analysis. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.Īmplification of a variety of human genomic amplicons from low to high GC content using Q5 High-Fidelity DNA Polymerase. High-Fidelity DNA Polymerase is unlike typical, The addition of the 5X Q5 High GC Enhancer. (≥ 65% GC), amplification can be improved by (1X) reaction concentrations and is recommendedįor most routine applications. Q5 Reaction Buffer contains 2 mM Mg ++ at final High-Fidelity DNA Polymerase is supplied withĪn optimized buffer system that allows robustĪmplification regardless of GC content. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-FidelityĭNA Polymerase is ideal for cloning and canīe used for long or difficult amplicons. Processivity-enhancing Sso7d domain to support ![]() With 3´→ 5´ exonuclease activity, fused to a Is a high-fidelity, thermostable DNA polymerase
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